Affiliation:        Postdoctoral Associate (May 2013 – present)

Biology Department, Rutgers University, Camden, New Jersey, 08102

Email :              vs383@camden.rutgers.edu

PROFESSIONAL BACKGROUND

Postdoc Associate May 2013 to present

Postdoc Fellow April 2012- May 2013

Dept. of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, USA

Visiting Scientist Nov.’ 2009 – Apr.’ 2012

The Wistar Institute, Philadelphia, USA. Laboratory of Dr. Jumin Zhou

EDUCATION

Ph.D. (2003 –2009), Awarded Nov.’ 2010, Embryo Biotechnology Lab, National Institute of Immunology, India.

Master of Science (Genetics) 2001- 2003

Department of Genetics, University of Delhi, New Delhi (India)

Bachelor of Science 1998- 2001

Sri Venkateswara College, University of Delhi, New Delhi (India)

PUBLICATION

“Enhancer blocking activity of the Insulator at the H19-ICR is independent of chromatin barrier establishment.” Vikrant Singh and Madhulika Srivastava, Molecular Cellular Biology, 2008, 28, 11, 3767-3775.

AWARDS AND SCHOLARSHIPS

Council of Scientific and Industrial Research (CSIR), India, Research Fellowship (2003-2008) for pursuing Ph.D.

• Qualified National Eligibility Test for Lecturer in Indian Universities.
• Student Conference Travel and Participation Award by Organizers of Chromatin Structure and Function, 2007
• University Gold Medal, University of Delhi in M.Sc. Genetics, 2003
• Monsanto PostGraduate Scholarship during M.Sc. Genetics (2002-2003)
• University of Delhi South Campus Scholarship (2001-2002) for meritorious performance in National Level M.Sc. Genetics Entrance Exam                                

TECHNICAL EXPERTISE

 Epigenetics and Molecular Biology:

• Chromatin Immunoprecipitation Assays: Crosslinked ChIP using crosslinked chromatin and Native ChIP with unfixed chromatin
• Experimental aspects of ChIP on chip (Chromatin IP followed by microarray hybridization)
• Real Time Quantitative PCR using SYBR Green
• DNA Methylation Analysis by bisulphite conversion, COBRA
• Recombinant DNA and molecular biology techniques: DNA and RNA analysis – isolation, cloning, PCR amplification, cDNA library preparation, expression analysis by Real Time qPCR, Allele specific expression analysis, Primer extension assay, Radioactive Southern and Northern Blotting, Western Blotting

Techniques in Drosophila biology – Whole embryo in-situ hybridization, Generation of Transgenic Flies, Basic Genetics, Handling and maintenance of fly populations

Cell Culture: Culturing of Mouse ES cell lines, Establishment and maintenance of Primary Mouse Embryonic Fibroblasts – PMEF’s. Maintenance and transfection of several mammalian cell lines like NIH3T3, HeLa, HEK293T, RetropakPT67.

Experimental Animal Handling: Experience of Mouse Genetics, Maintaining colonies of transgenic mice, basic mice handling and breeding set up, Genotyping mice by PCR using tail biopsy.

Microscopy: Basic light and fluorescence microscopy